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Open Access Original article

SOFI-based 3D superresolution sectioning with a widefield microscope

Thomas Dertinger12*, Jianmin Xu1, Omeed Foroutan Naini1, Robert Vogel1 and Shimon Weiss134*

Author Affiliations

1 Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA

2 SOFast GmbH, Dresdener Str 14, 10999, Berlin, Germany

3 Department of Physiology University of California Los Angeles, UCLA, Los Angeles, USA

4 California NanoSystems Institute University of California Los Angeles, UCLA, Los Angeles, USA

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Optical Nanoscopy 2012, 1:2  doi:10.1186/2192-2853-1-2

Published: 25 April 2012

Abstract

Background

Fluorescence-based biological imaging has been revolutionized by the recent introduction of superresolution microscopy methods. 3D superresolution microscopy, however, remains a challenge as its implementation by existing superresolution methods is non-trivial.

Methods

Here we demonstrate a facile and straightforward 3D superresolution imaging and sectioning of the cytoskeletal network of a fixed cell using superresolution optical fluctuation imaging (SOFI) performed on a conventional lamp-based widefield microscope.

Results and Conclusion

SOFI’s inherent sectioning capability effectively transforms a conventional widefield microscope into a superresolution ‘confocal widefield’ microscope.