Original article
SOFI-based 3D superresolution sectioning with a widefield microscope
1 Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA
2 SOFast GmbH, Dresdener Str 14, 10999, Berlin, Germany
3 Department of Physiology University of California Los Angeles, UCLA, Los Angeles, USA
4 California NanoSystems Institute University of California Los Angeles, UCLA, Los Angeles, USA
Optical Nanoscopy 2012, 1:2 doi:10.1186/2192-2853-1-2
Published: 25 April 2012Abstract
Background
Fluorescence-based biological imaging has been revolutionized by the recent introduction of superresolution microscopy methods. 3D superresolution microscopy, however, remains a challenge as its implementation by existing superresolution methods is non-trivial.
Methods
Here we demonstrate a facile and straightforward 3D superresolution imaging and sectioning of the cytoskeletal network of a fixed cell using superresolution optical fluctuation imaging (SOFI) performed on a conventional lamp-based widefield microscope.
Results and Conclusion
SOFI’s inherent sectioning capability effectively transforms a conventional widefield microscope into a superresolution ‘confocal widefield’ microscope.
